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Preservation methods were evaluated for jerusalem artichokes (Helianthus tuberosus L.) to facilitate the back-up of fi eld collections. In vitro plant cultures were established from fi eld-harvested tubers for nine jerusalem artichoke cultivars obtained from the Plant Gene Resources of Canada, Saskatoon Research Center, Saskatoon, Canada. Most cultivars could be maintained at 5 °C or under 25 °C growth room conditions for at least 6 months. Excised shoot tips from in vitro cultures were cryopreserved using plant vitrifi cation solution 2 (PVS2; 15% w/v ethylene glycol, 15% w/v dimethyl sulfoxide, 30% w/v glycerol, and 13.7% w/v sucrose in half strength media salts) as a cryoprotectant. PVS2 exposures of 15 or 30 min at 0 °C resulted in an average regrowth of 34% across fi ve jerusalem artichoke cultivars after liquid nitrogen exposure. Jerusalem artichokes can be successfully maintained using both reduced temperature and cryopreservation approaches. Jerusalem artichoke (Helianthus tuberosus L.) is a cold-hardy North American wild rela- tive of the sunfl ower, Helianthus annuus L. Jerusalem artichoke plants were spread from Saskatchewan east to Ontario and south to Arkansas and Georgia by Native Americans who cultivated them for edible tubers that can be baked, steamed, or boiled. Raw tubers can be sliced or shredded for salads. The tubers contain high levels of inulin, which has been pursued as a bulking agent for artifi cial sweet- eners (McLaurin et al., 1999). Since jerusalem artichoke plants produce large quantities of biomass with few inputs, this crop has also been considered for silage production (Seiler, 1993). Furthermore, Helianthus breeders have interest in jerusalem artichoke germplasm since Sclerotinia sclerotiorum (Lib.) resistance has been identifi ed through tissue culture screening (Cassells and Walsh, 1995). The USDA National Plant Germplasm System maintains seven clonal accessions of H. tuberosus; however, 165 H. tuberosus (L.) accessions are propagated using tubers at Plant Gene Resources of Canada. These accessions are not currently distributed or listed on the Germplasm Resources Information Network- Canadian version (GRIN-CA) database. Since the tubers must be dug and replanted every 3 years, jerusalem artichoke plants are relatively high maintenance compared to many seed- propagated species. Labor costs and fi eld space also make maintaining duplicate cultivars at separate locations prohibitively expensive. This research was performed to identify

Preservation Methods for Jerusalem Artichoke Cultivars