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A set of 216 PCR-based molecular markers was screened for polymorphisms using two morphologically dissimilar broccoli lines, `VI-158' and `BNC'. Fifty-nine of these markers, representing 69 detected polymorphisms and two morphological markers, were used to construct a genetic linkage map of broccoli [ Brassicaoleracea  (L.) var.  italica ] from a population of 162 F 2:3  families generated from the cross between these two lines. Ten genetic linkage groups were generated that spanned a distance of 468 cM with an average interval width of 9.4 cm. This map represents the first combined SSR and SRAP map of  Brassica oleracea . Comparisons are made to existing maps of  Brassicanapus  and to inter-specific maps of  Brassicaoleracea . To our knowledge this is the first linkage map of broccoli [ Brassicaoleracea  (L.) var.  italica ] and should provide a useful tool for the genetic analysis of traits specific to the  italica  subspecies.

(261) A PCR-based Linkage Map of Broccoli [Brassica oleracea(L.) var. italica] and Comparisons with Existing BrassicaMaps