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Noni,  Morinda citrifolia , is receiving a lot of attention for its potential medicinal effects. Hawaii is an ideal growing environment for this plant, where it has been used for many purposes, including medicinal ones, by ancient Polynesians. Currently, there is a rapidly developing noni industry in the state of Hawaii. Propagation of this plant is almost exclusively by seeds, and germination generally requires a couple of months without preconditioning or about a month if mechanically scarified. We developed an in vitro protocol that significantly improves percent germination rate by altering incubation temperature and the in vitro culture basal medium. Germination time was decreased to 4 days when the embryo was extracted and exposed to 31 °C. A basal medium containing 1/2 Murashige and Skoog (M&amp;S) salts was the most effective in reducing germination time and increasing percent germination. Stem pieces obtained from in vitro-propagated seedlings produced callus when explanted in 1/2 M&amp;S containing various levels of naphthalene acetic acid (NAA). The most effective treatment was 0.5 μ m  NAA and the least effective treatment was 2 μ m  NAA. Treatments without NAA did not produce callus. Calli treated with 4.40 μ m  6-benzylaminopurine (BA) or 8.80 μ m  BA were the most effective in promoting caulogenesis. We also demonstrated that the number of first generation seedlings produced from each embryo could be increased by treatment with 8.80 μ m  BA.

(279) Morinda citrifolia In Vitro Seminal Germination, Callus and Organogenesis Induction