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Pear scab caused by  Venturianashicola is one of the most important diseases of oriental pear. Breeding a variety resistant to scab can be improved through marker-assisted selection (MAS). Bulked segregant analysis (BSA) and amplified fragment length polymorphic (AFLP) analysis were performed to identify DNA markers linked to the scab-resistant gene ( Vn ) using a population from a cross between PS2-93-3-98 (resistant parent) and Yali (susceptible parent). A total of 480  Eco R I/ Mse I primer combinations were used to identify markers specific to PS2-93-3-98 and resistant pool. Three AFLP markers linked to  Vn , E-AGT/M-CCA 245 , E-ATT/M-CCG 300 , and E-GGT/M-TCT 225 , were selected. Linkage analysis between the selected markers and  Vn  locus was conducted with 51 individual plants. The selected markers, E-AGT/M-CCA 245 , E-ATT/M-CCG 300 , and E-GGT/M-TCT 225 , were located at 3.9, 3.8, and 1.2 cm away from  Vn , respectively. For practical application, we are currently converting selected markers to simple PCR-based markers. The markers could be used to increase selection efficiency in pear-breeding programs for scab resistance.

(272) DNA Markers Linked to the Resistance Gene of Scab in Oriental Pear (Pyrus sp.)