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RAPD and AFLP markers were first used to construct a molecular map in a BC1 red raspberry population consisting of 70 individuals that segregated for Phytophthora root rot resistance. RAPD markers linked to root rot resistance were identified by bulk segregant analysis and through QTL anlaysis. Two common genomic regions were identified by both analyses and were estimated to explain ≈50% of the phenotypic variation. RAPD markers flanking the QTL were cloned and made into sequence specific markers for potential use in marker assisted selection. In addition to the linked markers, RAPDs spread throughout the linkage map were also sequenced and developed into either SCARs, CAPs, or codominant SSRs. Attempts were made to locate red raspberry resistance gene analogs using degenerate primers designed on conserved regions encoding known resistance genes. Results on the type and map position of identified RGA's and selection efficiency of linked markers analyzed in red raspberry cultivars of characterized root rot resistance will be discussed.

Mapping Phytophthora Root Rot Resistance in Red Raspberry using SCARs, CAPs, AFLPs, and RGAs