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Acreages of `Nules' Clementine and `Afourer' mandarin have increased rapidly in California. One way to produce seedless mandarins is using Navel oranges or Satsuma mandarins as buffer to prevent cross-pollination. In order to determine the number of necessary buffer rows or spacing to prevent cross-pollination, we used AFLP markers to determine the pollen parentages of `Nules' and `Afourer' seedlings from two sites. The AFLP markers were able to identify Clementine as pollen parents of 26.6% (25/94) of the `Afourer' seedlings from one site. The pollen of Clementine was able to travel across minimum of 32 rows to pollinate `Afourer' mandarins. We found 12.73% (14/110) of the `Afourer' seedlings at the east side of the site were progenies of `Minneola' tangelo. Pollen of `Minneola' was able to travel across minimum 94 rows to pollinate `Afourer' mandarins. 12.73% (14/110) of the `Afourer' seedlings at the east end of the site were progenies of Clementine. Pollen of Clementine was able to travel minimum 54 rows to pollinate the `Afourer' mandarin at the east end of the site. The AFLP markers also identified `Afourer' mandarin as pollen parents of almost all `Nules' seedlings (98.63%, 72/73) at a second site. The pollen of `Afourer' was able to travel across minimum of 74 acres of empty ground from the east or minimum of 91 rows of Navel (128 acres) from the north to pollinate `Nules' Clementine. The results showed how far can compatible pollens traveled to cause seeds in mandarins in California. The implication from the results in seedless mandarin production in California is discussed.

Determination of Pollen Parentages of Mandarin Seedlings Using AFLP Markers and Implications for Seedless Mandarin Production in California