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The bacterial isopentenyl transferase ( ipt ) gene involved in cytokinin biosynthesis was fused with a promoter from the serine proteinase inhibiter ( win3.12 ) gene of  Populus x generosa  and introduced into  Nicotiana tabacum  (cv. Havana). Transformants were confirmed by PCR reaction and Southern blot analysis, and then analyzed for phenotypic characteristics. RT-PCR analysis detected transcripts of the ipt gene following the wounding of  win3.12:ipt  transgenic plants. In  win3.12: ipt  transgenic plants, lateral shoot number and the diameter of lateral branches that developed following apical shoot removal increased relative to wild-type plants. However, the increase in cytokinin production following wounding appeared to be short lived. The potential utility of this construct in enhancing lateral branching in ornamental crops will be discussed.

Wound-inducible Expression of the ipt Gene Stimulates Enhanced Lateral Shoot Development in Tobacco