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In vitro rooting and subsequent greenhouse survival of `Autumn Britten', `Boyne', `Comet',`Nova' and `Qualicum' raspberry ( Rubus idaeus  L.) plantlets were compared following four weeks on a rooting medium with and without activated charcoal, and with 0.1, 0.5, 1.0, 2.0 or 3.0 milligrams per litre IBA. The addition of charcoal significantly increased the percentage of plantlets that produced roots in vitro for the hard-to-root cultivars. Percent rooting in vitro was highest with the three lower levels of IBA. Root number was influenced only by the cultivar, while root diameter and length were affected by all the factors investigated. Greenhouse survival was affected by the cultivar, the presence or absence of charcoal and the IBA level in the in vitro rooting medium, with significant interactions. Provided charcoal was present in the rooting medium, the level of IBA didn`t alter survival. The addition of charcoal to the rooting medium improved greenhouse survival of the three hardest-to-root cultivars. Plug plant stem length; internode length and dry weight were increased by the presence of charcoal in the in vitro rooting medium for all but the easiest to establish cultivar. Chemical names used: 3-indolebutyric acid (IBA).

Rooting Medium Composition and In Vitro Rooting and Greenhouse Survival of Five Raspberry Cultivars