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Field-grown mustard greens,  Brassica juncea , were used to validate several observations of a greenhouse study which reported nutrient changes in mustard greens grown, in part, under ambient and reduced light. The cultivar Florida Broadleaf was transplanted into a Hildago sandy clay soil near Weslaco, Texas (26° 08' Lat.) on 6 Nov 2003. Greens were fertigated with 30 kg·ha -1  of N on 1 Dec. Plants 14 days before harvest were grown under the following four light regimes: (1) continuous ambient light; (2) 7 days of 50% shade then 7 days of ambient light; (3) 7 days of ambient light then 7 days of 50% shade; and (4) 14 days of 50% shade. Cumulative solar light was 28.9 and 19.4 kW/m 2  during the first and second 7 days, respectively. Measured cumulative light, as PPFD, for treatments 1-4 were 108, 67, 78, and 44 m m ·m -2 ·s -1 , respectively. Plants were harvested at 0800, 1100, and 1400 h on 2 Jan. 2004. Shade during the last 7 days generally evoked the greatest responses. Increased shade duration did not significantly effect the agronomic performance, but did increase leaf total carotenoids, chlorophylls, water content, and reduced total ascorbate levels. As time of daylight progressed, sample plant weight and average leaf weight decreased in shaded plants only. Free ascorbic acid, chlorophyll a:b ratio, and the chlorophyll to carotenoid ratio decreased with time of day. Cumulative sunlight, as PPFD, was significantly correlated with total ascorbate (fresh weight basis), chlorophyll a:b ratio, and plant weight ( P  &lt; 0.06) and negatively correlated with chlorophylls and total carotenoids (dry weight basis). Thus, cloudy weather prior to harvest can reduce leaf Vitamin C and alter leaf greenness

Preliminary Observations on the Effect of Light Intensity and Time of Day on Mustard Greens Leaf Ascorbic Acid and Greenness at Harvest