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Leaf explants of Coffea arabica cv. Rubi, 'Catuai Vermelho 81ʼ, and 'IAPAR 59ʼ were placed onto MS basal medium with 4 µM of picloram for 8-12 weeks in the dark at 27 ± 2 °C (standard temperature). Explants ('Rubiʼ and 'Catuai Vermelho 81ʼ) with callus were transferred to a SP basal medium plus 0, 12, 24, and 48 µM BAP, whereas for 'IAPAR 59ʼ, only 48 µM BAP was added. After 8 weeks for 'Rubiʼ and 'Catuai Vermelho 81ʼ and after 12 weeks for 'IAPAR 59ʼ, all cultivars showed indirect embryogenesis. Embryogenic material was transferred to a SP basal medium without BAP, in light (16-hour photoperiod) and standard temperature. In a second experiment, leaf explants belonging to all three cultivars were inoculated in petri dishes with SP basal medium plus 0, 2.5 and 5 µM BAP, in the dark at standard temperature. After 8 weeks, only 'IAPAR 59ʼ produced direct embryos. These were transferred to SP basal medium. About 30% of embryos developed abnormalities. Normal embryos were successfully transferred to soil.

Somatic Embryogenesis from Three Coffee Cultivars: `Rubi', `Catuaí Vermelho 81`, and `IAPAR 59'