English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Softwood shoots can be forced from sections of branches of trees or from basal stems of shrubs by cutting into ≈40-cm lengths and placing these segments horizontally in flats filled with perlite. We have had our best success using stems that are &gt;1.5 cm in diameter. Although the best environment that we have found for producing the most and longest softwood shoots is under intermittent mist, this is unacceptable for producing explants because of microbial contamination. Rather, for micropropagation, watering must be done two to three times a day and care must be taken to avoid water spray onto the stem segments or the subsequent softwood growth. Irrigation can be by hand or by using drip irrigation. For trees, using the basal portions of large branches allows for selection of shoots from within the “cone of juvenility.” Theoretically, these should propagate better than shoots taken from the outer, more adult portions. Although late winter through spring are the best times for forcing, some shoots will grow if the stem sections are harvested nearly any time of the year, except for October through December in southern Illinois.

691 Obtaining Shoot Explants From Softwood Shoots Forced From Large Stem Segments of Woody Plants