English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Garlic ( Allium sativum  L.) belongs to the Alliaceae family and originated from Asia and Mediterranean countries. Their bulblets are rich in starch and aromatic substances. The rate of garlic propagation in field conditions takes several years for the production of a certain number of seed bulbs for the release of a new variety. The use of tissue culture techniques is a useful tool for overcoming this problem. The aim of this work was to increase the mean number of shoots derived from the meristem isolation and to verify the percentage of callus formation and to analyze vigor of the material. The initial meristems were inoculated in a salt and vitamin B5 media except for the iron element, which was provided by MS medium added to in mg·L -1 :  myo -inositol (100.0), nicotinic acid (1.0), piridoxine (1.0), thiamine (10.0), sucrose (20.0 g·L -1 ), agar (6.0 g·L -1 ). BAP and TDZ were added at: 0.0; 1.0; 1.5; 2.0; and 2.5 μM This material remained in a growth room for a 16-h photoperiod, radiation of 20 μMol·m -2 ·s -1  and 25 °C for 40 days. Although `Sao Marcos' produced more vigorous shoots, no significant difference was found for the mean number of shoots. `Sao Valentim' cultivar shows more callus at the shoot base, making this cultivar more prone to somaclonal variation On the other hand, BAP estimulates the appearance of callus, but it has been shown that this is cultivar-dependent.

334 Meristem Isolation of Garlic (Allium sativum L.) Cultivars Sao Marcos and Sao Valentim