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Garlic (cv California Late) was produced under four irrigation regimes (110% and 130% evapotranspiration with two water cut-off dates, 10 and 24 May 1999) in combination with three nitrogen fertilization levels (100, 250, and 400 lb total N). Bulbs were manually harvested mid-June, cured 3 weeks shaded at ambient temperatures and the outer whorl of cloves manually peeled. Samples were freeze-dried, and carbohydrate (fructan and free sugars) and alliin (substrate for alliinase activity and indicator of potential pungency) concentrations were determined by HPLC. The percent dry weight was not affected by the irrigation treatment, but was reduced with increased N rate (41.3% to 39.0%). Alliin concentrations varied from 8.3 to 13.8 mg/g DW for 110% and 130% Et o  irrigation treatments. Alliin concentrations were not affected by N fertilization (average = 11.5 mg/g DW). Fructan concentrations were affected by N fertilization treatment, with the highest content (802 mg/g DW) associated with the lowest N level, and the lowest (717 mg/g DW) content in samples from the highest N rate. Sucrose concentrations increased with increased N, but glucose and fructose concentrations did not vary with N fertilization. Fructan as percent of total carbohydrate remained constant across irrigation treatments (96.6% + 0.2%) and across N fertilization treatments (96.6% + 0.3%).

216 Carbohydrate and Alliin Concentrations in Garlic Produced under Different N Fertilzation and Irrigation Regimes