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Storage of  Banksia menziesii  pollen was assessed at 20, 4, –20, –80, and –196 °C using a semisolid medium of 1% agar, 15% sucrose, 0.01% boric acid, 0.03% calcium nitrate, 0.02% magnesium sulfate, 0.01% potassium nitrate, and an incubation temperature of 25 °C. The germination percentage remained constant at ≈70% in all treatments except for pollen stored at 20 °C, which exhibited only 25% germination after 6 months. Pollen viability was assessed using fluorescein diacetate (FDA), but the results did not reflect the loss of germinability at 20 °C and correlation with in vitro results was variable. Floret position on the inflorescence did not affect germination, but pollen viability varied over the flowering period with maximum germination at midseason.

Storage Temperature Affects Viability of Banksia menziesii Pollen