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Stem cuttings of greenhouse-grown gardenia ( Gardenia jasminoides  Ellis) were surface-sterilized and planted on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L (BA) and 0.5 mg/L (NAA). This study examines the effects of pH and various sugars on the growth and proliferation of in vitro-cultured gardenia. The highest average shoot number and shoot length were obtained at pH 4.0 to 5.0. In determining the effect of sugars on shoot proliferation, our findings indicated that sucrose and glucose at 30 and 40 g/L, respectively, produced a higher shoot number when compared to fructose and xylose. In addition, sucrose and glucose produced the highest root number, root length, and rooting percentage, while fructose and xylose had no effect on rooting. Sucrose and fructose produced more calli in comparison to other sugars. Xylose at 40 g/L produced the highest dry weight (18.5%), while xylose at 10 g/L produced the highest fresh weight (94.4%).

Shoot Proliferation and Growth of Gardenia (Gardenia jasminoides Ellis) in Response to pH and Sugar Concentrations in Vitro