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Terminal stem cuttings of  Kalmia latifolia  were collected from wild plants (Milford, N.H.) on 12 Nov. and transported on ice to Orono, Maine, for analysis. Samples were processed as follows: 1) stems wrapped in dry cheesecloth; 2) stems wrapped in moist cheesecloth; and 3) stems seeded with crushed ice and wrapped in moist cheesecloth. Prepared samples were subjected to freezing tests to a low temperature of –36C. Following two weeks of incubation at 21C, samples were evaluated for leaf, petiole, stem, and vegetative bud damage. Evaluation of frozen samples revealed: 1) stem tissue remained undamaged to –36C; 2) leaf damage was inconsistent across all handling methods, with no clear LST estimate, and ice seedinggenerally resulted in increased tissue damage; 3) LSTs for vegetative buds and petiole bases were –18C and –15C, respectively, and both yielded definitive and consistent results across all treatments. The results indicate bud and petiole tissue to be the best to use for future studies on LST estimates in  Kalmia latifolia .

LOWEST SURVIVAL TEMPERATURE (LST) ESTIMATES FOR KALMIA LATIFOLIA (L.) AS AFFECTED BY NUCLEATION/SEEDING TREATMENTS