English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The use of buffalograss [ Buchloe dactyloides  (Nutt.) Engelm] in home lawns and golf courses has been increasing because of its drought resistance and low growth habit. In vitro regeneration of buffalograss at a high frequency may provide an effective tool to introduce new variation for breeding use. The positive effects of AgNO 3  on friable embryogenic callus production and regeneration efficiency is well documented in maize. In order to determine if AgNO 3  has the same effect on buffalograss, two vegetatively propagated cultivars, a female `609' and a male `45-3' were tested at three different concentrations of AgNO 3  at 5, 10, and 15 mg·L –1  using immature inflorescences as explants. Murashige and Skoog medium supplemented with 2 mg 2,4-D/L was employed as the control medium. Medium containing AgNO 3  significantly promoted the production of friable callus for `45-3' with the highest percentage achieved at 10 mg AgNO 3 /L. AgNO 3  medium led to production of significantly larger calli than found for the control. However, no difference was detected among 5, 10, and 15 mg AgNO 3 /L with regard to the callus formation ability and the size of callus initiated on these three treatments. Calli were then transferred to MS medium supplemented with BA at 0.1, 0.5 or 1.0 mg·L –1  to induce shoot formation. BA at 0.5 mg·L –1  gave the best differentiation response. Calli formed in the absence of AgNO 3  produced more shoots per callus, but more calli were produced in the presence of AgNO 3 , and the overall regeneration efficiency was much higher with AgNO 3  at 10 mg·L –1 . In contrast, AgNO 3  showed no promotive effect on callus production and regeneration for `609'.

AgNO3 Promotes Callus Production and Regeneration of Immature Buffalograss Inflorescence Culture