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Previous studies indicated that the number of shoots formed per nodal explant varied significantly depending upon the type of culture container used. Amount of media per container and amount of time that media was autoclaved were variables that differed among the containers. To determine the cause for the container effect on shoot number, a study was conducted in which autoclave time and amount of media per container were the same for all tested containers. Media was autoclaved in 500-mL batches for 30 min, then poured in 30-mL aliquots into sterile containers. Containers tested were plastic petri plates, GA-7 Magenta vessels and glass jars. Depth of the medium was 5 mm in plates, 8 mm in vessels, and 12 mm in jars. Nodes from in vitro grown shoots of `Triumph', `Regale', and `Fry' were cultured on Murashige and Skoog salts and vitamins with 2 mg·L –1  BA and 8 g·L –1  agar. Results indicated that the greatest number of shoots formed in the jars. In a second study, nodes were cultured on petri plates containing 30 mL of medium (depth 5 mm) or 70 mL (depth 12 mm). Two to three times more shoots formed on the plates with the greater amount of medium. These studies indicate amount and depth of medium are factors influencing shoot number.

Effect of Quantity and Depth of Media per Culture Container on Shoot Production of Muscadine Grape Cultured in Vitro