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Actively growing shoots from  Pulmonaria  L. `Roy Davidson' were cultured in vitro on Murashige and Skoog medium containing benzyladenine (BA) to establish proliferating cultures. BA at 0, 0.4, 0.8, 4.4, 8.8, and 44.4 μ m  was compared for shoot proliferation and rooting response. Shoot count was highest on 8.8 μ m  BA with root count highest on 0 or 0.4 μ m  BA. Subculture 4 weeks later of shoots to the same treatments resulted in highest shoot counts on 44.4 μ m  BA. Optimum level for micropropagation was 8.8 or 44.4 μ m  BA. Greatest rooting was at 0 or 0.4 μ m  BA.

Axillary Shoot Proliferation from in Vitro Culture of Pulmonaria L. Shoots