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Reactive O 2  species produced when electron transport is disrupted have been implicated in several environmental stress-induced disorders. Superoxide (  \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{O}_{2}^{-}\) \end{document}  ) is produced at two or more sites in mitochondria isolated from bell pepper fruit supplied with succinate and NADH. SOD and KCN completely inhibited   \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{O}_{2}^{-}\) \end{document}   production with both substrates. Antimycin A inhibited   \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{O}_{2}^{-}\) \end{document}   production with succinate, but not with NADH. Insensitivity of O 2  uptake to KCN increased in mitochondria isolated from bell peppers stored at 2C and their   \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{O}_{2}^{-}\) \end{document}   production increased with NADH as substrate, but decreased with succinate. Disrupting the mitochondrial membranes enhanced   \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{O}_{2}^{-}\) \end{document}   production with NADH and reduced production with succinate. Greater   \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{O}_{2}^{-}\) \end{document}   production with NADH may result from the inability to transfer electrons from NADH through the alternative path. The KCN-insensitive alternative path in some plant tissues appears to reduce the potential production of   \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{O}_{2}^{-}\) \end{document}  .

Sites of Superoxide Production in Mitochondria Isolated from Bell Pepper Pericarp Tissue