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Scanning electron microscopic (SEM) studies and gravimetric analysis of in vitro cultured leaf surfaces showed reduced epicuticular wax (EW) structurally and quantitatively as compared to greenhouse plants. However, leaves of in vitro plantlets subjected to polyethylene glycol-treatment (PEG) showed an increase in quantitative and structural EW which was similar to that of greenhouse plants. Furthermore, leaves initiated during in vitro culture and which persisted, when transferred to the greenhouse, showed an increase in structural wax as well as in amount, 30 days after transplanting in the greenhouse. Similarly, leaves newly-formed in the greenhouse from in vitro cultured plants developed more dense crystalline structure and greater levels of wax than those leaves observed immediately after removal from culture. A correlation between density of structural EW and amount of EW were observed in in vitro cultured, PEG-treated in vitro cultured and greenhouse grown leaves.

807 PB 141 LEAF EPICUTICULAR WAX OF IN VITRO GRAPE PLANTLETS TREATED WITH AND WITHOUT POLYETHYLENE GLYCOL