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Immature embryos or cotyledons were cultured at three stages of development (PF 1 =3, 30-60, or 100) from two unrelated apricot genotypes. Explants were cultured on MS medium supplemented with 2.4-D (0 or 1 μM) and either BA or TDZ (0, 0.5. 5.0 or 20 μM). Stage 1 embryos cultured on MS media without hormones tended to form embryoid-like structures. Regeneration was highest with stage 2 cotyledons on media containing between 5-20 μM TDZ and 1.0 μM 2.4-D; shoot morphology was abnormal at the highest level of TDZ. In another factorial experiment, stage 2 cotyledons were cultured on media containing TDZ (six levels, 0 to 20 μM) in combination with either 2.4-D (0 or 1 μM) or IBA (0, 1, or 5 μM), Regeneration of normal shoots was highest on the medium containing 5 μM IBA and no TDZ. Regenerated shoots were transferred to woody plant medium (WP) containing 3% sucrose and supplemented with 6 μM 2iP and 2.2 μM BA to promote stem elongation. Shoots were transferred to WP media supplemented with NAA or IBA to induce rooting. Culture on media containing 10 μM IBA in total darkness induced rooting in less than two weeks.

579 PB 082 FACTORS AFFECTING REGENERATION AND ROOTING OF APRICOT SHOOTS DERIVED FROM IMMATURE EMBRYOS