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Studies were completed to optimize Stage II production efficiency of  Pontederia cordata,  a native wetland plant. Basal shoot tips from established cultures were subcultured into 60 ml glass culture tubes, 155 ml glass baby food jars, 350 ml GA7 polypropylene vessels or 500 ml clear polypropylene tissue culture containers containing full strength Linsmaier and Skoog mineral salts and organics supplemented with 3.0% sucrose, 2.0 mg/liter benzyladenine, 1.0 mg/liter indole-3-acetic acid, 50 mg/liter citric and ascorbic acids solidified with 8 g/liter TC® agar. Shoot tip to medium volume (ml) ratio was maintained 1:10 in each culture vessel. Vessel type had no significant effect on either shoot quality or multiplication rate (9.5 shoots/shoot tip/28 days). A maximum production efficiency of 1216 shoots/ft 2 /28 days was achieved using GA7 vessels. Stage II shoot multiplication rate significantly decreased when the interval between subculture exceeded 28 days.

042 EFFECTS OF VESSEL TYPE AND SUBCULTURE DURATION ON IN VITRO MULTIPLICATION OF PONTEDERIA CORDATA L.