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Leaf callus of  Ipomoea cordatotriloba  was initiated by culturing explants on Linsmaier and Skoog medium containing 3 g yeast extract/liter, 18.9 μ m  ABA, 2.3 μ m  2,4-D, and 0.15  m  sucrose. Calluses were transferred to Murashige and Skoog media containing 17.8 μ m  BA and 0, 1, 10, or 100 μ m  PCIB. The number of shoots from calluses grown on medium containing 10 μ m  PCIB increased significantly, and the percentage of calluses exhibiting shoot regeneration almost doubled compared to calluses grown on regeneration medium without PCIB. Protoplasts isolated from stem and petiole tissues of in vitro-grown plants were cultured in Kao and Michayluk 8p medium to the callus stage. Calluses (4-6 mm) were transferred to the callus induction and regeneration media used to regenerate leaf-explant callus. Of the protoplast-derived calluses cultured on media containing 10 or 100 μ m  PCIB, ≈13% and 18%, respectively, regenerated shoots after 2 months; none regenerated on the medium without PCIB. Chemical names used: abscisic acid (ABA); 2,4-dichlorophenoxyacetic acid (2,4-D); N 6 -benzyladenine (BA); α -p-chlorophenoxyisobutyric acid (PCIB).

PCIB Enhances Regeneration of Ipomoea cordatotriloba Dennstedt Leaf Explants and Protoplasts