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Chlorophyll "a" fluorescence (Fvar) was compared with respiration and vitamin C content of broccoli (Brassica oleracea L. (Botrytis group)) during storage at 1C. The amplitude of the Fvar maxima declined in a similar manner as respiration and vitamin C content. Fvar was highly correlated with respiration ( r = 0.83, P > = 0.0001). The correlation of Fvar with vitamin C content was weaker ( r = 0.42, P > = 0.0002). The results demonstrate that Fvar is an indicator of postharvest changes in broccoli and that Fvar can be used as a nondestructive indicator of early changes in tissue condition (i.e., degree of freshness) of broccoli in storage.

Chlorophyll Fluorescence as a Nondestructive Indicator of Freshness in Harvested Broccoli