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Susceptibility of tomato ( Lycopersicon esculentum  Mill) genotpyes to the root-knot nematode  Meloydogyne incognita  and to heat stress can be evaluated in a single labor- and time-saving operation using a nondestructive  in vitro  excised root technique. Seeds are sterilized and germinated for 2 days on 1% water agar. Five-mm root sections are grown at 28 and 35 C for 30 days on Gamborg-B medium with and without nematode inoculum. Evaluation criteria include fresh and dry weight and the appearance of juveniles, adults, gulls, and egg masses. Evidence will be presented on the breakdown of resistance to  M. incognita  under high temperature stress.

AN IN VITRO TISSUE CULTURE TECHNIQUE FOR EVALUATING SUSCEPTIBILITY TO NEMATODE AND TOLERANCE TO HEAT STRESS IN TOMATOES