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Use of wild species for in vitro sweetpotato improvement has been limited, in part, by the lack of suitable regeneration systems for these species. Shoot regeneration in 4 closely related species,  I. batatas, I. cordatotriloba, I. trifida  and  I. triloba , were evaluated. Callus was initiated using methods described by Otani and Shimada (1988). Calli were transferred to regeneration media containing 17.75 uM BAP and 0, 1, 10 and 100 uM PCIB. Organogenesis was enhanced by the presence of PCIB. With  I. cordatotriloba  calli grown on media with 10 uM PCIB, a 2-fold increase in the percentage of calli exhibiting shoot regeneration was observed as compared to calli grown on media with BAP alone. A significant increase in the average number of shoots per callus was also observed. The other species examined appeared to be less sensitive than  I. cordatotriloba  to the PCIB treatments.

ENHANCEMENT OF SHOOT REGENERATION BY PARACHLOROPHENOXY ISOBUTYRIC ACID (PCIB) IN LEAF CULTURES OF IPOMOEA SPECIES IN THE SECTION BATATAS.