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Somatic embryogenesis of coffee has been studied for the purpose of obtaining target tissues for stable genetic transformation through use of a particle gun. Eight cultivars of  C. arabica  were selected for callus induction from leaves. Primary calli were induced within two weeks in over 98% of the leaf disks explanted on MS medium with 2,4-D and kinetin prior to treatment on a secondary culture medium. Somatic embryos were obtained from `Catuai' and `Blue Mountain' after six months from explanting. Somatic embryos were germinated in MS media and developed into plants. Somatic embryos and embryogenic calli are being used for gene transformation experiments with the helium-driven particle gun.

DEVELOPMENT OF TRANSFORMATION SYSTEM FOR COFFEA ARABICA USING PARTICLE GUN METHOD