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Hypocotyl sections of cauliflower were cultured in horizontal, upright or inverted positions on Murashige and Skoog medium with different concentrations of 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA). Inverted position gave the highest differentiated score and greatest fresh weight of the two month-old tissues, followed by upright and horizontal positions respectively. Combination BAP and NAA caused synergistic effect on tissues. The best treatment for getting callus was combination of 5 mg/liter BAP, 2 mg/liter NAA and upright position, whereas for getting tissue differentiation was combination of 5 mg/liter BAP, 1 mg/liter NAA and inverted position.

HYPOCOTYL ORIENTATION AND HORMONE INFLUENCE IN VITRO REGENERATION OF ROOT AND SHOOT OF CAULIFLOWER (BRASSICA OLERACEA L. VAR. BOTRYTIS) `PUAKEA'.