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Commercial strains of Agaricus bisporus (Lange) Imbach that were grown in vitro at 32C for 4 to 5 weeks on a medium containing 250 µg amantadine/ml followed by hyphal tip-culturing showed an enhanced tolerance of ammonia in the compost during cropping trials. Yield of mushrooms by a treated off-white strain (GSP-18) and a treated white strain (Marlowe-503) was higher in composts with elevated levels of ammonia (0.11% to 0.27%) than that of the untreated cultures. Tolerance was related to the ability of the treated cultures to grow vegetatively in the ammonia-enriched compost. Tolerance was expressed by the cultures for at least 14 months following induction.

Tolerance to Ammonia in the Common Edible Mushroom Induced in Vitro