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Biotin- and fluorescein-labeled probe has been used to map. specific sunflower rDNA sequences by  in situ  hybridization on mitotic chromosomes of  Allium cepa, Allium fistulosum  and interspecific hybrid derivatives, There are three hybridization sites in  A. cepa  and more than six in an interspecific triploid. This  in situ  hybridization technique offers new cytogenetic markers useful in the construction of a physical genomic map of  Allium  and offer a means to document introgression of these genomes.

CHOMOSOMAL LOCATION OF BIOTIN- AND FLUORESCEIN-LABELED DNA BY IN SITU HYBRIDIZATION IN ALLIUM.