Molecular study of Mitochondrial MT-TL1Leucine gene and some Biochemical parameters in association with Coronary Heart Disease in patients attending Erbil cardiac center

The present study was carried out between December 2013 and April 2014, in Hawler cardiac center. Sixty patients with coronary atherosclerotic patients were enrolled in our study (40 males and 20 females), their age ranged between 41-78 years, in addition, 30 healthy individuals (15 males and 15 females) were enrolled as control, their ages ware ranged from 36-75 year. The statistical analysis showed significant difference at the level of 0.05  between coronary atherosclerotic patients and normal healthy control in terms of the mean of diabetic (for females 169.200 ± 16.510 and males 179.150 ± 13.252 mg/dl in coronary atherosclerotic patients, for females 97.733 ± 2.289 and males 94.200 ± 3.806 mg/dl in normal healthy groups respectively), triglycerides (for females 190.650 ± 12.117 and males 202.750 ± 12.105 mg/dl in coronary atherosclerotic patients, for females 108.866 ± 6.677 and males 144.733 ± 9.087 mg/dl in normal groups respectively), total cholesterol (for females 207.750 ± 10.685 and males 217.400 ± 9.571 mg/dl in coronary atherosclerotic patients, for females 107.600 ± 4.182 and males 170.466 ± 8.487 mg/dl in normal groups respectively), low density lipoprotein (for females 132.100 ± 6.307and males 141.475 ± 5.809 mg/dl in coronary atherosclerotic patients group, for females 98.666 ± 4.744 and males 123.866 ± 1.166 mg/dl in health groups respectively) and high density lipoprotein(for females 37.750 ± 1.798 and males 34.575 ± 1.349 mg/dl in coronary atherosclerotic patients group, for females 44.260 ± 1.462 and males 47.066 ± 1.318 mg/dl in health groups respectively) (biochemical testes) (P<0.01). Percentage between coronary atherosclerotic patients and healthy control in smoking (life style) shown, most of coronary atherosclerotic patients (52%) were nonsmoker, while (48%) were smoker. Also, there was a percentage in patients with hypertension (62%) were had hypertension, while (38%) were had normality blood pressure. The results of MT-TL1 Leucine UUR gene sequencing were clearly showed no polymorphic mutation to be linked with regards to the disease status or development with those published in the NCBI database, indicating that this gene is not responsible and it is not counted as marker for this disease in our study.