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To provide guidelines for collecting and analyzing urinary, salivary, and plasma melatonin, thereby assisting clinicians and researchers in determining which method of measuring melatonin is most appropriate for their particular needs and facilitating the comparison of data between laboratories. The benefits and disadvantages of current methods of collecting and analyzing melatonin are summarized. Although a single method of analysis would be the most effective way to compare studies, limitations of current methods preclude this possibility. Given that the best analysis method for use under multiple conditions is not established, it is recommended to include, in any published report, one of the established low threshold measures of dim light melatonin onset to facilitate comparison between studies. Administration of melatonin with time of day and midnight are fitted with bivariate normal distribution and the probability density function, marginal distributions and corresponding expectations are obtained using stochastic model. Here we suggest that saliva sampling is a good method to measure.

A bivariate mathematical model for the effect of melatonin production on samplings