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This study presents a methodology for the study of superficial pollutant dispersion with a fluorescent tracer 'Rodamine B' dye in natural water bodies. Here are presented many practical details to help researchers and students who wish to work with ‘Rodamine B’ dye, but do not have any experience on the subject. The radiance measuring method on aerial photos was chosen over the ‘in situ’ fluorescence method, and the two methods are compared. In order to adapt the method to the area and provide the case study, data acquired during field work (1997 and 1998) in the southernmost part of the Patos Lagoon estuary were used. This methodology consists of generating superficial dye paths and monitoring their dispersion with aerial photographs and sequential water sampling, in orthogonal transects over each generated dye path. The real and continuous curves of ‘Rodamine B’ dye concentrations are obtained by statistical correlation between the absorbance measured in water samples and the radiance level extracted from aerial photos for the same points. This study describes several types of chemical fluorescent tracers, their application and choice, how to prepare ‘Rodamine B’ dye solution in the laboratory, as well as the care required in transport and field use. It also shows, step by step, planning the experiment, equipments and infrastructure needed, tracer delivery form, water sampling and the acquisition of aerial photographs, as well as later treatment and analysis of data obtained. In future, using same data, we intend to study the determination of eddy diffusion coefficients and validation of a mathematical model of dispersion. Key-Words: Rodamine B, pollutant dispersion, chemical tracers, fluorescent tracers, oceanic tracers, estuary, Patos Lagoon.

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Metodologia para Utilização do Traçador Fluorescente ?Rodamina B? no Estudo da Dispersão Superficial, Adaptada para Aplicação no Estuário da Lagoa dos Patos