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In order to reduce the impact of dams, engineering proposes to build fish passages, mechanisms which allow local fish migration. Fish ladders are the most popular type of fish passage built to provide passage upstream.. They consist in a hydraulic channel with slots for energy dissipaEstabelecimento de Parâmetros Hidráulicos para Escadas de Peixes do Tipo Ranhura Vertical Baseados em Características de Espécies Neotropicais 112 tion in order to create specific flow to meet the needs of fish. Thus, this flow should have specific values of maximum velocity and turbulence. In the vertical slot fish ladder, the slope and slot width are the main factors to change the flow velocity and the fish ladder length. On the other hand, these constructive factors depend on the fish swimming capability. Based on biometric aspects of three neotropical species, slot width values were obtained. The same procedure was performed to obtain the maximum velocity values using data on the swimming capability of these species. It was concluded that additional studies on South American fish fauna should be performed to define these fish ladder parameters.