English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Aims: A local fungal isolate, Aspergillus niger USM F4 produced high level of mannanase activity when cultivated in a shallow tray system (45 x 40 x 7 cm3) using palm kernel cake (PKC), an easily available cheap agricultural waste which are found abundantly in Malaysia. Methodology and Results: A range of 0.25 to 1.5 cm bed heights were investigated in tracking in the most suitable condition and maximum production of mannanase. The highest mannanase production of 918.68 U/g substrate was obtained on the fifth day of cultivation after using all the optimised cultural conditions that consisted of 400 g of PKC that equivalent to 0.50 cm of substrate thickness with the particle size of ≤ 0.5 mm, moisture content of 80% (w/w) with the addition of 2% (w/w) molasses as a carbon source and 4% (w/w) ammonium nitrate as a nitrogen source, inoculums size of 1x107 spores/ml, with once at every 24 h of mixing frequency and cultivation temperature at room temperature 30±2 °C. Conclusion, significance and impact of study: The results obtained from this study showed that a shallow tray system was suitable to be used for getting highest enzyme production in SSF. Besides using a bigger volume of substrate, the correct substrate bed height is also important.

Mannanase Production by Aspergillus niger USM F4 Via Solid Substrate Fermentation in a Shallow Tray Using Palm Kernel Cake as a Substrate