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Aims: Rotavirus (RV) is the most important etiological agent of diarrhea in children with high morbidity and mortality, mainly in developing countries. A big number of children from one to five year old have been infected by RV, with at least one type of virus. Data show that human body can be infected repeatedly by different strains of RV. It is predicted that there exist 16 types of G genotypes of RV. This study was aimed to identify the G genotypes of RV in children with diarrhea in Pekanbaru, Indonesia. Methodology and results: This research is a cross-sectional study involving children aged zero to sixty months from January to July 2015. The stool samples were collected from diarrhea patients and the identification of RV was done by using the rapid test. The RNA isolation was performed on positive isolates. The identification of the G genotypes was performed by using a semi nested reverse transcription PCR method. The study involved 71 children with diarrhea. VP7based RV detection showed 47 positive samples (66.1%). The predominant G type of the positive results are G1, G9 and G3 namely 36.2%, 25.5% and 12.8%. The average age of the subjects was 15.3±2.3 months. The majority of the subject were females (53.6%). Most of the diarrhea feces presented in this study were neither haemorrhagic nor liquefied. The subjects in this study are also presented with co-symptoms such as fever (100.0%), gastrointestinal disorder (94.6%) and dypsnea (16.1%). Conclusion, significance and impact study: Based on this study, we conclude that the predominant G types is G1. This study was performed to identify the predominant G genotype of RV and designed the antigen-antibody based in diagnose RV.

malaysian journal of microbiology

Genotyping the G types of rotavirus and its clinical presentation in children under five years old with diarrhea in the government clinics in Pekanbaru, Indonesia