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The growth and protease production by Bacillus sp. (SBP-29) was examined for poultry processing industries. The maximum protease activity was 3028 U/mL using 1.5% (w/v) of soybean meal as substrate. Soybean meal is an inexpensive and readily available, thus it can be used as the cost effective crude material for the production of an extracellular protease. Inorganic nitrogen sources proved to be less favorable, for protease production as strong catabolic repression was observed with ammonium ions. A maximum of 3208 U/mL of protease was produced in 18 h in a 10L bioreactor. The enzyme has temperature and pH optima of 60°C and 9.5 respectively. However, the temperature stability range is from 20-90 °C and pH stability range is from 6.0–12.0. The protease was completely inhibited by phenylmethylsulfonyl fluoride (PMSF) and diodopropyl fluorophosphate (DFP), with little increase (10-15%) in the production of upon addition of Ca and Mg.

Enhanced productivity of serine alkaline protease by Bacillus sp. using soybean as substrate