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When a phospholipids bilayer with a metallochromic indicator was measured by total internal reflection with thermal lens spectrometry, the signal was more fluctuated than that of a dye solution. To clarify the difference of the signal fluctuation for the bilayer and the dye solution, power spectra of the results were obtained by a Fourier transform. In a lower frequency region, the intensity of the power spectrum for the phospholipid bilayer measurement was higher than that for the dye solution measurements. We supposed that the fluctuation was caused by the heterogeneity of the bilayer, or aggregation of the dye molecules in the bilayer, or both. Signals at different temperatures were obtained, and these power spectra were compared with each other. The spectra lower than 0.05 Hz increased as the temperature became higher, and agreed with the expected result.

Fluctuation of Thermal Lens Signal in Total Internal Reflection Configuration from Phospholipid Bilayer