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The flow profile of electroosmosis in capillary-zone electrophoresis was observed using a microscope-CCD-video system, rectangular capillary (50 x1000?) and fused-silica capillary (75 ? in diameter). A rhodamine 6 G-methanol solution was used as a fluorescent sample solution. The front zone of electrosomosis forms a reverse parabolic flow profile. The flow speed at the center of a cross-section of capillary was 0 .4% lower than that at the near the wall. The progress of frontal zone while an applying pulsed electric field was observed. It was found that the growth and decay is very rapid , less than 1/15 second (which is equal to the time resolution in this experiment) . 電気浸透流及び圧力差流の先端ゾーンプロフィルを顕微鏡下で観察した.長方形断面のキャピラリー管(50×1000μm)と円形断面の溶融石英キャピラリー管(直径75μm)を用い,色素としてローダミン6Gを使用しその蛍光を観察した.電気浸透流の管断面方向での速度分布は,管中央部が管壁近傍に比べて約0.4%遅いことが分かった.電気浸透流は逆放物線状フロープロフィルを形成していることがいずれのキヤピラリー管中においても確認できた.電気浸透流の立ち上がり時間と立ち下がり時間は本実験の時間分解能(1/15秒)以下であり非常に迅速に達成されることを発見した

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New Developments in Capillary Electrophoresis. Development and Improvement of Capillary Electrophoresis. Formation of a reverse parabolic flow profile with electroosmosis in capillary zone electrophoresis and the behavior of zone progress related to the application of a pulsed electric field.