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Cobalt(II) is extracted into chloroform as the ternary complex consisting of cobalt(II), zincon and zephiramine cation. By the addition of pyridine, the quaternary complex of cobalt is extracted, of which molar absorptivity is about four times that of the ternary complex. The difference of absorbances of extracts of the quaternary and ternary complexes is proportional to the concentration of cobalt. Copper(II), nickel(II) and zinc(II) at 5, 3 and 2 times the amount of cobalt, respectively, do not interfere with the determination. Iron(III) and manganese(II) decompose zincon, and interfere seriously.

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Differential spectrophotometric determination of cobalt by using the extraction of ion-pair of cobalt-zincon chelate anion with zephiramine cation in the presence of pyridine.