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6-Methoxy-2-methylsulfonylquinoline-4-carbonyl chloride was found to be a highly sensitive fluorescence derivatization reagent for both primary and secondary alcohols in high-performance liquid chromatography. Its reactivity was investigated for 1-propanol, benzyl alcohol, cyclohexanol, and 1-hexanol. The reagent reacted with the alcohols or phenol in benzene in the presence of pyridine to produce the corresponding fluorescent esters, which could be separated on a reversed-phase column, Cosmosil 5C 18 -AR, with aqueous 70% (v/v) acetonitrile

analytical sciences

6-Methoxy-2-methylsulfonylquinoline-4-carbonyl Chloride as a Fluorescence Derivatization Reagent for Alcohols in High-Performance Liquid Chromatography