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There have recently been great demands to develop flow injection analysis (FIA) and other flow analysis methods for slow flow rate systems. Goto et al.l have employed an economical automated method using continuous micro flow for the determination of mercury in environmental analysis. Micro flow analysis stream (less than 100 μl min 1) is suitable for continuous monitoring because of the small amount of reagents required. We have also proposed an S4 system designed for the basic improvement of FIA instruments.2 Combinations of a narrow (i.d. sO.5 mm) and short (~10 cm) reaction tube, a small-volume flow cell (8 μl) and small flow rate (50.05 ml min 1) gave better sensitivity for the determination of macromolecules in biotechnology assays. Very recently, we have applied this system as a capillary stream sensor to the determination of potassium ion.3 Moreover, as biosensing devices have become smaller", stable pumping at a very small flow rate would be strongly desired through FIA use. However, it is not easy to buy such a stable pump commercially, although various pumping mechanisms are designed for the improvement of FIA apparatus. We therefore present a double plunger micropump with linear cam mechanism having very short plunger strokes, in order to attain higher sensitivity and precision in S4-FIA determination.

A Double-Plunger Micropump for Small Flow Rate Down to Several il/min in Flow Injection Analysis