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A label-free fluorescent probe based on autofluorescent hyperbranched poly(amide amine) (HPAMAM) for copper ions was designed. HPAMAM is a cationic polymer containing many amino groups, which could bind Cu 2+ ions to form cupric amine complexes, leading to a selective quenching of the fluorescence intensity of HPAMAM via inner filter effect. The fluorescence intensity of HPAMAM decreased with increasing concentration of Cu 2+ ions and the linear response ranged from 0.05 to 25 μM (R 2 = 0.995), with the corresponding detection limit (3σ/k) of 17.15 nM. The HPAMAM fluorescent probe provided a simple, rapid, selective and sensitive fluorometric method for detecting Cu 2+ ions, which could be also applied for detection of Cu 2+ ions in real water samples.

Autofluorescent Hyperbranched Poly(amide amine) as Effective Fluorescent Probe for Label-free Detection of Copper(II) Ions