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A simple colorimetric immunoassay for quantification of human immunoglobulin G (hIgG) is herein described. The assay is based on the aggregation inhibition of silver nanoparticles (AgNP) functionalized with hIgG antibody (anti-hIgG) on the surface. The aggregation is measured in terms of attenuance values ratio at 400 and 530 nm (A 400 /A 530 ). A linear response between A 400 /A 530 and hIgG concentration is observed in the range 25 - 200 ng mL -1 , and the detection limit is estimated as 11 ng mL -1 hIgG.

Immunoassay for Human IgG Using Antibody-functionalized Silver Nanoparticles