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2-Hydroxytryptanthrin (T2OH) and its sodium salt (sodium tryptanthrin-2-olate, T2ONa) were synthesized as near-infrared (NIR) dyes for fluorescent imaging. The absorption maxima (λ(a,max)) of T2OH under a pH range from 1.3 to 7.2 and from 8.5 to 10.6 were ca. 410 nm and ca. 495 nm, respectively. Moreover, the fluorescence maxima (λ(f,max)) were ca. 660 nm regardless of the pH range. T2ONa was water soluble and the λf,max were ca. 660 nm in both aprotic and protic solvents.

Photophysical Properties of the 2-Hydroxytryptanthrin and Its Sodium Salt as Near-infrared Dyes for Fluorescent Imaging