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The preparation and application of a stop and go extraction tip (StageTip) used for pre-purification of sample solutions for LC-MS/MS analysis of DNA adducts was simplified and improved to increase throughput while maintaining high adduct selectivity. It was demonstrated that the StageTip composed of two sheets of a poly(styrene-divinylbenzene) copolymer disk could be easily prepared and proved useful for selective extraction of trace amounts of DNA adducts from a sample solution containing a great quantity of normal deoxynucleosides.

Facile and Effective Pretreatment Using Stop and Go Extraction Tips for LC-MS/MS Analysis of Trace Amounts of DNA Adducts