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A capillary electrophoresis method coupled with electrochemiluminescence detection for the analysis of vinorelbine (VNB) in the urine of tumor patients was established in this research. Complete determination of VNB was achieved in 8 min using a background electrolyte of 50 mmol/L phosphate buffer (pH 9.0) and a separation voltage of 15 kV. The calibration curves showed a linear range from 2.8 × 10(-10) to 1.6 × 10(-8) mol/L. The relative standard derivation for VNB was below 3.4%. The linear relationships were good and the correlation factor of VNB exceeded 0.985. The detection limits were 1.0 × 10(-11) mol/L under the optimal conditions. The developed method was applied to the sensitive determination of VNB in the urine of tumor patients.

analytical sciences

Rapid and Sensitive Detection of Vinorelbine in the Urine of Tumor Patients by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II)-based Electrochemiluminescence Assay