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A vitamin U-bonded stationary phase was prepared and the retention behavior of inorganic anions was examined using ion chromatography.  Inorganic anions were retained on the vitamin U-bonded stationary phase under acidic as well as neutral eluent conditions in the ion-exchange mode.  The elution order of the examined anions under neutral eluent conditions was nearly the same as that observed in common ion exchange mode, while the elution order observed under acidic eluent conditions was completely different from that observed in common ion exchange mode.  The retention of the analyte anions under the neutral eluent conditions was due to the sulfonium groups of the vitamin U, while protonated primary amino groups caused retention of the analyte anions with different selectivity under acidic conditions.  The retention factor of the analyte anions increased with decreasing eluent concentration under both eluent conditions.  The present system was applied to the determination of bromide and nitrate contained in seawater.

Vitamin U-bonded Stationary Phase in Capillary Ion Chromatography