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A kinetic fluorometric method was proposed for the quantitative determination of epinephrine (EP) in human plasma samples with the aid of second-order calibration methods based on alternating trilinear decomposition (ATLD) and alternating fitting residue (AFR) algorithms. It was based on that EP could be gradually converted to a highly fluorescent intermediate product by an oxidation reaction, and further to a non-fluorescent degradation product (o-quinone). These methodologies fully exploit the second-order advantage of the employed three-way kinetic fluorescence data, allowing the concentrations of EP to be quantified even in the presence of uncalibrated interferences. The average recoveries obtained from ATLD and AFR with a factor number of 2 (N = 2) were 100.7 +/- 3.3 and 100.4 +/- 2.2%, respectively. In addition, elliptical joint confidence region (EJCR) tests as well as figures of merit (FOM) were employed to evaluate the accuracy of the two algorithms.

Quantitative Analysis of Epinephrine in Human Plasma Samples Using Kinetic Fluorometric Method Combined with Second-order Calibration